Attenuation Effect Of Single And Combined Treatment With...

Attenuation effect of caffein and/or nifedipine against selenite-induced cataractogenesis Abstract Objectives: The current study was performed to investigate the anti-cataractogenic effect of single and combined treatment with caffeine and nifedipine in sodium selenite induced cataract. Methods: Seventy five healthy albino Wistar rats were divided into 5 groups; the first group received intraperitoneal injection of saline and served as control, 2nd group received a single subcutaneous injection of 30 nmol/g body weights of sodium selenite to induce cataract. 3rd and 4th groups received 0.1 mg/kg of nifedipine and 5.15 Â µmoles of caffeine respectively, starting two days prior to the administration of selenite and continuing such treatment till the end of experiment. Last group got the combined treatments of nifedipine and caffeine in the same regimen. Animals were decapitated after 5, 15 and 25 days of the experiment. Results: The results indicated a very highly significant decrease (PË‚0.001) in total soluble lens protein and Na+-K+ATPase activity, a very highly significant increase (PË‚0.001) in calcium lens content in selenite cataract group. Also significant changes in the molecular weight and area under peaks estimated in profile of electrophoretic mobility of lens proteins were found in selenite group. All these changes reduced markedly in caffeine and nifedipine group. Conclusions: nutrients with antioxidant capabilities can potentially delay against these changes such